551 Salk Hall
3501 Terrace Street
Pittsburgh, PA 15261
Research Assistant Professor
Department of Oral Biology
School of Dental Medicine
- Post doctoral associate, Cellular and Molecular Biology, Mount Sinai Medical Center, NY (1995)
- Post doctoral fellow, Immunobiology, Yale University, CT (1991)
- PhD, All India Institute of Medical Sciences, New Delhi (1989)
1) Regulation of DSPP and DMP1 functions- My long-term interests are in defining how structural elements present in these proteins modulate bio-mineralization and cellular processes. The SIBLING proteins, DSPP and DMP1, are important for mineralization in dentine and bone, respectively. While phosphorylation of these proteins is being studied extensively, the potential effects of these proteins to multiple signaling inputs in a cellular context are not completely understood. Specifically, we will examine how these signaling events shape up SIBLING proteins stability, modifications and functions in osteoblasts and odontoblasts.
2) Characterization of bioactive phospholipids that are important for mineralization and for signaling in osteoblasts and odontoblasts- Lipids, intra- and extracellular, play an important role in both physiological and pathological processes. While phospholipids have been suggested to play a role in mineralization, how they initiate and promote mineral growth is lacking. Detailed information on the lipid composition at a local level as well as the location of biochemical reactions is crucial for understanding their roles in bone cells function and dysfunction. In extending my research on the lipid messenger IP3-gated calcium signaling over the last ten years, we will now examine the location and actions of these signaling lipids in osteoblasts and odontoblasts using cellular and molecular approaches.
Jayaraman T, Bannon L, Beniash E, and Sfeir C. (2012). Intracellular mechanisms of phosphorylation in osteoblasts and odontoblasts. Volume 2: Bone and Dentin Phosphorylated Extracellular Matrix Proteins, Chapter 5 (Editor, M. Goldberg).
Jayaraman T, Duan Y, Zhang X, Day B, and Sfeir C. (2012). Differential processing and phosphorylation of DMP1 in cells of mineralized versus non-mineralized tissues (Cells Tissues Organs, Revised).
Hong W, Pang-Ning T, Jayaraman T, Shinsuke I, Li J, Bannon L, Huang H, Close J and Sfeir C. (2011) Dentin matrix protein 1 (DMP1) signals via cell surface integrin. Journal of Biological Chemistry, 286:29462-9
Deshpande AS, Fang PA, Zhang X, Jayaraman T, Sfeir C, Beniash E (2011) Primary Structure and phosphorylation of Dentin Matrix Protein 1 (DMP1) and Dentin Phosphophoryn (DPP) uniquely determine their role in biomineralization. Biomacromolecules, 12: 2933-45.
Jayaraman T, Tejero J, Chen BB, Blood AB, Frizzell S, Shapiro C, Tiso M, Hood BL, Wang X, Zhao X, Conrads TP, Mallampalli RK, Gladwin MT (2011). 14-3-3 binding and phosphorylation of neuroglobin during hypoxia modulate six to five heme pocket coordination and rate of nitrite reduction to nitric oxide. Journal of Biological Chemistry, 286:42679-42689.
Jayaraman T, Paget A, Shin YS, Li X, Mayer J, Chaudhry H, Niimi Y, Silane M, Berenstein A. (2008). TNF alpha mediated inflammation in cerebral aneurysms: A potential link to growth and rupture. Vascular Health Risk and Management, 4, 805-17.
Malathi K, Li X, Krizanova O, Ondrias K, Sperber K, Ablamunits V and Jayaraman T (2005). Cdc2/Cyclin B1 interacts with and modulates IP3 receptor (type 1) functions. Journal of Immunology, 175:6205-6210.
Jayaraman T, Berenstein V, Li X, Mayer J, Silane M, Shin YS, Niimi Y, Gunel M, Berenstein A (2005). Tumor necrosis factor alpha is a key modulator of inflammation in cerebral aneurysms. Neurosurgery, 57:558-64.
Jayaraman T, Marks AR (2000). Calcineurin is downstream of the inositol 1,4,5-trisphosphate receptor in the apoptotic and cell growth pathways. Journal of Biological Chemistry, 275: 6417-20.
Jayaraman T, Marks AR (1997). T cells deficient in inositol 1,4,5-trisphosphate receptor are resistant to apoptosis. Molecular Cell Biology, 17: 3005-12.
Jayaraman T, Ondrias K, Ondriasova E, Marks AR (1996). Regulation of the inositol 1,4,5-trisphosphate receptor by tyrosine phosphorylation. Science, 272: 1492-94.
Jayaraman T, Ondriasova E, Ondrias K, Harnick DJ, Marks AR (1995). Inositol 1,4,5 trisphosphate receptor is essential for T cell receptor signaling. Proceedings of National Academy of Sciences, 92: 6007-6011.